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In order to fully understand the mechanisms underlying tissue regeneration, 3D cell culture models are required. We present a novel technique for the fabrication of scaffold-based 3D cell models. The technique is based on polymer-conjugated dendrimers that bind themselves to the scaffold and can be used for permeabilisation of the scaffold, thus allowing the cells to colonise the scaffold. This technique is not limited to cell culture models involving the use of only one scaffold type, but can be used with any material that can be wetted. We demonstrated the use of this technique for two different scaffold types, providing proof of concept. We also demonstrated the superiority of the technique by demonstrating enhanced cell growth on the scaffold and a change in gene expression.Sunday, June 25, 2012
We have three main fabric shops in Neston. One locally, one in Wales and one in Cheshire. Some of these shops carry Milliner's Gloves and in particular, Mark & Ayla's of Cheshire. We have been buying gloves from Mark & Ayla for years. They are a small design and manufacturing business who have focused on producing quality leather goods. Since being in business they have been buying Milliner's Gloves from us and have been thrilled with the quality of the gloves that they have received.
Here is a glimpse of some of the gloves they make.
They also make a range of handmade tweed accessories including scarves and hats.
Recently Mark & Ayla have been sourcing new, high quality milliner's 0b46394aab